منابع مشابه
Human Adenine Phosphoribosyltransferase
Human adenine phosphoribosyltransferase has been purified 33,000-fold from erythrocytes to a specific activity of 9.58 pmoles of AMP formed per mg of protein per min. The native enzyme has a molecular weight of 34,000 and is composed of three subunits of equal molecular weight which appear to be associated by noncovalent forces. The highly purified enzyme is maximally active over a broad pH ran...
متن کاملAdenine phosphoribosyltransferase deficiency
Key-words Disease name and synonyms Definition Excluded diseases Diagnosis criteria Differential diagnosis Prevalence Molecular defect Clinical description Management including treatment Etiology Diagnostic methods Antenatal diagnosis Unresolved questions References Abstract Adenine phosphoribosyltransferase (APRT) catalyzes the synthesis of AMP (adenosine monophosphate) from adenine and 5'-pho...
متن کاملKinetic studies of adenine phosphoribosyltransferase.
The mechanism of reaction of adenine phosphoribosyltransferase from Ehrlich ascites tumor cells was investigated kinetically by initial velocity and product inhibition studies. The data are consistent with a mechanism in which 5-phosphoribosylpyrophosphate reacts with the enzyme to form an intermediate, presumably an enzyme-ribose 5-phosphate complex, with which adenine then reacts to form aden...
متن کاملAdenine Phosphoribosyltransferase from Monkey Liver
The structural requirements for the binding of purines to adenine phosphoribosyltransferase from monkey liver were explored by the determination of the K; values for 67 purines and purine analogues. Adenine was bound 40 times better than the most effectively bound analogues. Substitutions on the 6-amino group of adenine decreased binding to varying degrees. The hydroxylaminoand hydrazinopurines...
متن کاملAdenine Phosphoribosyltransferase Deficiency: A Rare Cause of Recurrent Urolithiasis
Background: Recurrent urolithiasis is troublesome for both patient and clinician, and in most cases, an underlying cause is not found. An important and underdiagnosed cause is adenine phosphoribosyltransferase (APRT) deficiency that gives rise to 2,8-dihydroxyadenine (2,8-DHA) stones. If diagnosed early, patient morbidity as well as the financial cost of treating stone recurrence can be avoided...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44140-9